The Ohio State University Zebrafish Facility is located in Bevis Hall in an independent environmentally controlled space containing approximately 1,300 tanks of various sizes, and areas for experimentation, food production, cleaning and storage. Aquaculture systems support rearing of zebrafish from fertilized embryos to adults. The facility currently houses numerous wildtype lines and dozens of mutant and transgenic lines. A separate system for the importation of zebrafish lines from universities worldwide is also located in the facility. All phases of zebrafish husbandry are carried out by Zebrafish facility staff and members of the Henion, Beattie and Jontes labs. NIH grant P30-NS045758 partially funded a major expansion of the facility.