1. Generation of transgenic mice by pronuclear microinjection
• Standard microinjection of purified DNA (transgene) into mouse embryos (strain FVB or C58BL/6)
• YAC/BAC Transgenesis
• Lentivial transgenesis
      
       Transgenic pups             Lentivial transgenesis
            
2. Knockout service
• Microinjection of ES cells into mouse blastocysts
• Gene targeting & morula aggregation (...coming soon)
  
   
    Knockout chimeric mice
  
  
3. Rederivation
• Rederive infected mouse lines from live animals to Specific Pathogen free (SPF)
• Rederive mouse lines from frozen embryos to SPF
4. Cryopreservation
• Cryo-preservation of mouse sperm
• Cryo-preservation of mouse embryos
• Embryos at 8-cell stage
• Embryos at 2-cell stage (Speed Cryo): Cryo-preserve 2-cell embryos generated from in vitro fertilization (IVF)
5. Assisted Reproduction
• In Vitro Fertilization (IVF)
• Ovary Transplantation (trial)
6. Other Services
• Consultation (free of charge):
• Strategy and preparation guidelines in initiative of transgenic services
• Genotyping transgenics: strategies and trouble shooting
• Cryo-preservation strategies and planning
• Colony management: breeding strategies, and assisted reproduction
• Microinjection of DNA into embryos from mouse strain specified by PI
• Generation of transient transgenics
• Vasectomy
• Ovariectomy
  
  
  

  

Contact Xin-an Pu for requistions or questions before scheduling any of the services.