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The Imaging Core has a Zeiss Axiophot for both bright field and epifluorescent imaging. The microscope is equipped with the objectives and filter cubes listed in the tables below. A Spot RT-KE Slider digital camera and MetaVue software are available on the Axiophot. This equipment allows the capture of high-resolution full color and monochrome images. Click here for a detailed description.
OBJECTIVES
|
Mag |
Correction |
NA |
Oil/Dry |
Phase/DIC |
|
2.5x |
Plan-neofluar
∞/- |
0.075 |
Dry |
No |
|
10x |
Plan-neofluar
∞/0.17 |
0.3 |
Dry |
Phase Contrast
(PH1) |
|
20x |
Plan-neofluar
∞/0.17 |
0.5 |
Dry |
No |
|
40x |
Plan-neofluar
∞/0.17 |
0.75 |
Dry |
No |
|
100x |
Plan-neofluar
∞/0.17 |
0.7-1.3
(collar) |
Oil |
DIC
(DIC 0.5-1.4) |
FILTER CUBES
|
Filter Name |
Open |
Texas Red |
TRITC |
DAPI |
FITC |
|
Example Fluorochromes |
Bright Field |
Texas Red, Alexa 568, Alexa 594 |
Rhodamine,
Alexa 546 |
DAPI |
FITC,
Alexa 488 |
|
