Confocal

The Leica TCS SL is the Imaging Core's confocal system and has these unique features.  Click here for a detailed description.

• In place of excitation filters, our confocal system has an acousto-optical tunable filter which allows:
  • Allows for independently attenuating each laser line
  • Less Crosstalk with multi-color stainings
  • Less bleaching by optimized excitation intensity
  • Fast switching - While the laser is scanning, it can be turned on in one spot and in the next spot it can be turned off
  • Region-of-interest Scan
    • Sample protection
    • Bleaching experiments (FRAP, FRET)
    • Activation/uncaging
      
      
• In place of emission filters, our confocal system has spectral detectors
  • No limitations caused by filter characteristics
  • Stepless adjustable spectral emission bands
  • Adjust detection bandwidth manually or with pre-sets

Microscope

The microscope used with this system is the Leica DM IRE2.  It is completely electronic and equipped with the following objectives and filter cubes.

OBJECTIVES

Name	Numerical Aperture	Resolution at 520nm (mm)	Working Distance	Coverslip thickness	Immersion Medium	Condenser Phase Annulus	Objective DIC Prism	Condenser DIC Prism	Correction Collar
100x HCX Plan Apo CS oil	0.70-1.40	0.45-0.23	90 mm	0.17mm	oil		D	63/100	Numerical Aperture
63x HCX Plan Apo CS oil	0.6-1.40	0.52-0.23	100mm	0.17mm	oil		E	63/100	Numerical Aperture
40x HCX Plan Apo CS oil	0.75-1.25	0.42-0.25	100mm	0.17mm	oil		E	40/63	Numerical Aperture
20x HC Plan Apo CS multi-imm	0.70	0.45	260mm		oil/ glycerol/ water		C	20	Immersion Medium
40x HCX Plan Fluotar Ph2	0.75	0.24	400mm	0.17mm	dry	Ph2			none
10x HC Plan Fluotar Ph1	0.30	1.06	11mm		dry	Ph1			none

FILTER CUBES

STANDARD
Label	Technical name	Excitation	Dichroic	Emission	Fluorochrome Examples
GRN	I3	BP 450-490	510	LP 515	Alexa 488, Cy2, YFP
RED	N2.1	BP 515-560	580	LP 590	Alexa 546, TRITC, Cy3
FAR	Cy5	BP 590-650	660	BP 663-738	Alexa 647, Cy5, TOTO-3
OTHER
CFP	Cyan GFP	BP 426-446	455	BP 460-500	CFP